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KMID : 0352720230470010044
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Journal of Ginseng Research
2023 Volume.47 No. 1 p.44 ~ p.53
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Comparative transcriptome and metabolome analyses of four Panax species explore the dynamics of metabolite biosynthesis
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Koo Hyun-Jin
Lee Yun-Sun
Nguyen Van Binh
Giang Vo Ngoc Linh
Koo Hyun-Jo
Park Hyun-Seung
Mohanan Padmanaban
Song Young-Hun
Ryu Byeol
Kang Kyo-Bin
Sung Sang-Hyun
Yang Tae-Jin
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Abstract
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Background: The genus Panax in the Araliaceae family has been used as traditional medicinal plants worldwide and is known to biosynthesize ginsenosides and phytosterols. However, genetic variation between Panax species has influenced their biosynthetic pathways is not fully understood.
Methods: Simultaneous analysis of transcriptomes and metabolomes obtained from adventitious roots of two tetraploid species (Panax ginseng and P. quinquefolius) and two diploid species (P. notoginseng and P. vietnamensis) revealed the diversity of their metabolites and related gene expression profiles.
Results: The transcriptome analysis showed that 2,3-OXIDOSQUALENE CYCLASEs (OSCs) involved in phytosterol biosynthesis are upregulated in the diploid species, while the expression of OSCs contributing to ginsenoside biosynthesis is higher in the tetraploid species. In agreement with these results, the contents of dammarenediol-type ginsenosides were higher in the tetraploid species relative to the diploid species.
Conclusion: These results suggest that a whole-genome duplication event has influenced the triterpene biosynthesis pathway in tetraploid Panax species during their evolution or ecological adaptation. This study provides a basis for further efforts to explore the genetic variation of the Panax genus.
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KEYWORD
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Adventitious root, De novo assembly, Oxidosqualene cyclase, Panax species, Specialized metabolite profiling
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